\(\left(\frac{a-4}{a}\right)\left(\frac{\sqrt{a}-1}{\sqrt{a}+2}-\frac{\sqrt{a}+1}{\sqrt{a}-2}\right)=\left(\frac{a-4}{a}\right)\left(\frac{\left(\sqrt{a}-1\right)\left(\sqrt{a}-2\right)-\left(\sqrt{a}+1\right)\left(\sqrt{a}+2\right)}{\left(\sqrt{a}+2\right)\left(\sqrt{a}-2\right)}\right)\)
\(=\left(\frac{a-4}{a}\right)\left(\frac{a-3\sqrt{a}+2-a-3\sqrt{a}-2}{a-4}\right)\)
\(=\frac{-6\sqrt{a}}{a}=\frac{-6}{\sqrt{a}}\)